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使用双光束扫描电子显微镜从玻璃体生物样品制备薄片进行冷冻电子断层扫描

JoVE 신문
생화학

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JoVE 신문 생화학

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

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使用双光束扫描电子显微镜从玻璃体生物样品制备薄片进行冷冻电子断层扫描

DOI:

10.3791/62350-v

•

07:00 min

•

August 05, 2021

•

Claudine Bisson², Corey W. Hecksel⁴, James B. Gilchrist, M. Alejandra Carbajal, Roland A. Fleck

¹Centre for Ultrastructural Imaging,New Hunt’s House, Guy’s Campus, King’s College London, ²Department of Biological Science,Birkbeck College, University of London, ³Electron Bio-Imaging Centre,Diamond Light Source, Harwell Science and Innovation Campus, ⁴SLAC National Accelerator Laboratory,Stanford University

Chapters

00:04Introduction
00:48Screening Plunge Frozen Grids and Preparing for FIB-Milling
01:28On-Grid FIB Milling of Plunge Frozen Cells
04:18Results: Analysis of Cryo-ET Data from FIB-Milled Lamella
06:28Conclusion

Summary

자동 번역

使用聚焦离子束铣削从冷冻生物样品中产生玻璃体网格片，用于冷冻电子断层扫描。

Tags

Vitreous Lamellae Cryo-electron Tomography Plasmodium Falciparum Red Blood Cells Malaria Protein Complexes Cryo-FIB-SEM Organoplatinum Coating Ion Beam Milling

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