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Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
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Chapters
- 00:05Introduction
- 00:33Extraction of The Total Non-Covalent-Linked Lipids from Mycobacteria
- 02:13Mycolic Acid Extraction by Acid Methanolysis
- 03:26Thin Layer Chromatography (TLC) Analysis
- 04:57Results: TLC Analysis of the Lipid Composition in Different Species of Mycobacteria
- 07:15Conclusion
A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided.
