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Chapters
- 00:04Introduction
- 01:36Small Scale Ganglioside Extraction and Partial Purification
- 03:35Large Scale Ganglioside Extraction and Purification
- 05:52Ganglioside Resolution and Staining
- 08:02Results: Small and Large Scale Ganglioside Extraction and Partial Purification
- 09:18Conclusion
Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.
