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Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies
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Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

DOI:
10.3791/62392-v

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09:08 min

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May 24, 2021

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, , , ,
1Divisions of Pulmonary Medicine,Boston Children’s Hospital

Chapters

  • 00:04Introduction
  • 00:49Extraction of Mouse Lung
  • 03:34Precision Cut Lung Slices (PCLS)
  • 05:35Vasoconstrictor Experiment
  • 06:20Preparing the Tissue for RNA or Protein Lysis on PCLS
  • 07:26Results: Ex Vivo Pulmonary Vessel Structure and Contractility Studies of PCLS
  • 08:32Conclusion

Summary

자동 번역

자동 번역

Presented here is a protocol for preserving the vascular contractility of PCLS murine lung tissue, resulting in a sophisticated three-dimensional image of the pulmonary vasculature and airway, which can be preserved for up to 10 days that is susceptible to numerous procedures.

Tags

Keywords: Precision Cut Lung SlicesEx VivoPulmonary VesselContractilityStructureSpatial OrientationLive Surrogate ModelSignaling CascadesPulmonary Vascular DiseasesEuthanasiaTracheaRight VentricleHeparinPBSAgaroseVibratomeDMEM

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