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単一細菌への重水素取り込みの誘導ラマン散乱イメージングによる迅速抗菌薬感受性試験
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JoVE 신문 생물학
Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
DOI:

12:08 min

February 14, 2022

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Chapters

  • 00:04Introduction
  • 00:38Deuterium (D2O) Incorporation Treatment in the Presence of Antibiotics
  • 03:50Preparation of Bacteria in Urine Environment and Blood Environment
  • 05:42Stimulated Raman Scattering (SRS) Imaging of D2O Metabolic Incorporation in a Single Bacterium
  • 08:44Results: The Influence of Deuterium Incorporation on Bacterial Growth
  • 11:14Conclusion

Summary

자동 번역

このプロトコルは、D2O代謝の単一細胞刺激ラマン散乱イメージングによる2.5時間以内の迅速な抗菌剤感受性試験(AST)アッセイを提示します。この方法は、尿または全血環境の細菌に適用され、診療所での迅速な単一細胞表現型ASTに変化します。

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