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Chapters
- 00:04Introduction
- 00:43Isolation of Valve Cells
- 03:35In vitro Calcification Assay
- 04:23Results: Identification of Mouse Valve Cell Phenotype and Calcification Assay
- 05:02Conclusion
This article describes the isolation of mouse aortic valve cells by a two-step collagenase procedure. Isolated mouse valve cells are important for performing different assays, such as this in vitro calcification assay, and for investigating the molecular pathways leading to aortic valve mineralization.
