Journal
/
면역학 및 감염병학
/
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
JoVE 신문
면역학 및 감염병학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

DOI:
10.3791/62422-v

•

10:16 min

•

October 30, 2021

•

, , , , , ,
1São Carlos Institute of Physics,University of Sao Paulo

Chapters

  • 00:02Introduction
  • 01:44Luciferase Activity Assay
  • 03:53Cell Proliferation-Based MTT Assay
  • 04:27NS2B-NS3 Protease Activity Assay
  • 05:19NS5 RdRp Elongation Assay
  • 06:32Representative Results
  • 08:06Conclusions

Summary

자동 번역

자동 번역

In this work, we describe the protocols used in replicon-based and viral enzyme-based assays to screen for inhibitors of Zika virus replication in a high-throughput screening format.

Tags

Zika VirusAntiviral AssayHigh-throughput ScreeningRepliconViral EnzymeRenilla LuciferaseNS3 ProteaseNS5 RNA-dependent RNA PolymeraseRdRpBHK-21 Cell LineCell CultureTrypsinizationCompound ScreeningDMEMDMSOPositive ControlNegative ControlRenilla Luciferase Lysis Buffer

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

High-throughput Detection Method for Influenza Virus

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

A Protocol for Analyzing Hepatitis C Virus Replication

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Zika Virus Specific Diagnostic Epitope Discovery

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

Read Article