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Genoombrede analyse van histonmodificatieverdeling met behulp van de chromatine-immunoprecipitatie-sequencingmethode in Magnaporthe oryzae
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Genoombrede analyse van histonmodificatieverdeling met behulp van de chromatine-immunoprecipitatie-sequencingmethode in Magnaporthe oryzae

DOI:
10.3791/62423-v

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09:25 min

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June 02, 2021

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, , , , , ,
1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy,Beijing University of Agriculture

Chapters

  • 00:04Introduction
  • 00:39Preparation of Protoplasts from M. oryzae
  • 01:48In Vivo Crosslinking and Sonication
  • 03:22IP of Crosslinked Protein/DNA
  • 04:23Collecting and Rinsing the IP Products
  • 05:04Elution and Reverse Crosslinking of Protein/DNA Complexes
  • 05:56Purification and Recovery of DNA
  • 06:51DNA Repair and Solexa Library Construction
  • 08:23Results: Chromatin Immunoprecipitation Sequencing Method for Genome-wide Histone Modification Analysi
  • 08:53Conclusion

Summary

자동 번역

자동 번역

Hier presenteren we een protocol om de genoombrede verdeling van histonmodificaties te analyseren, die nieuwe doelgenen in de pathogenese van M. oryzae en andere filamenteuze schimmels kunnen identificeren.

Tags

Keywords: Chromatin Immunoprecipitation Sequencing (ChIP-seq)Histone ModificationsMagnaporthe OryzaeEpigenetic RegulationFungal PathogenesisGenome-wide AnalysisTranscription FactorsDNA-protein InteractionsProtoplast PreparationSonicationAgarose Gel Electrophoresis

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