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用于硝酸盐和亚硝酸盐测量的大鼠骨骼肌匀浆的制备

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Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

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用于硝酸盐和亚硝酸盐测量的大鼠骨骼肌匀浆的制备

DOI:

10.3791/62427-v

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07:19 min

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July 29, 2021

•

Ji Won Park*¹, Samantha M. Thomas*¹, Lee J. Wylie, Andrew M. Jones, Anni Vanhatalo, Alan N. Schechter, Barbora Piknova

¹Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health, ²Sport and Health Sciences, College of Life and Environmental Sciences, St Luke’s Campus,University of Exeter

Chapters

00:04Introduction
00:31Preparation for Homogenization
01:50Homogenization
05:30Results: Nitrate and Nitrite Levels in Rat Skeletal Muscle Homogenates
06:54Conclusion

Summary

자동 번역

我们提出了三种不同方法的方案，用于大鼠骨骼肌组织的四个不同肌肉群的均质化，以测量和比较硝酸盐和亚硝酸盐的水平。此外，我们比较不同的样品重量，以研究组织样品大小是否会影响均质化的结果。

Tags

Rat Skeletal Muscle Nitrate Nitrite Homogenization Stop Solution Potassium Ferricyanide N-methylmaleimide Surfactant Centrifugation Methanol Bead Homogenization Pulverizer Homogenization

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