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从诱导多能干细胞生成3D全肺类器官，用于模拟肺发育生物学和疾病

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Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

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从诱导多能干细胞生成3D全肺类器官，用于模拟肺发育生物学和疾病

DOI:

10.3791/62456-v

•

09:45 min

•

April 12, 2021

•

Sandra L. Leibel^2,3, Rachael N. McVicar³, Alicia M. Winquist³, Evan Y. Snyder^2,3

¹Department of Pediatrics,University of California, San Diego School of Medicine, ²Sanford Consortium for Regenerative Medicine, ³Sanford Burnham Prebys Medical Discovery Institute

Chapters

00:04Introduction
01:05Definitive Endoderm Induction from Induced Pluripotent Stem Cells (Day 1-3)
03:13Anterior Foregut Endoderm (AFE) Induction and Lung Progenitor Cell (LPC) Differentiation
05:003D lung Organoid Induction (Day 16 22)
06:493D Lung Organoid Branching and Maturation
07:11Results: Characterization of Endoderm and 3D Whole Lung Organoids
09:04Conclusion

Summary

자동 번역

本文描述了诱导多能干细胞与含有近端和远端上皮肺细胞以及间充质的三维全肺类器官的逐步定向分化。

Tags

Keywords: 3D Whole Lung Organoids Induced Pluripotent Stem Cells Lung Developmental Biology Lung Disease Modeling Cryopreservation Genetic Manipulation Definitive Endoderm Induction ROCK Inhibitor GFR Basement Membrane Matrix

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