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通过 CLARITY 组织清除对完整神经元树突进行成像和定量

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Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

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通过 CLARITY 组织清除对完整神经元树突进行成像和定量

DOI:

10.3791/62532-v

•

07:45 min

•

April 20, 2021

•

Brandon T. Pekarek, Patrick J. Hunt², Benjamin D. W. Belfort², Gary Liu, Benjamin R. Arenkiel³

¹Department of Genetics and Genomics,Baylor College of Medicine, ²Medical Scientist Training Program,Baylor College of Medicine, ³Department of Neuroscience,Baylor College of Medicine

Chapters

00:04Introduction
00:45Tissue Clearing
01:57Preparing and Mounting the Cleared Tissue
04:19Imaging Cleared Tissue Samples
06:07Results: Imaging and Quantification of Neuronal Dendrites
07:22Conclusion

Summary

자동 번역

神经元树突形态通常是功能的基础。事实上，许多影响神经元发育的疾病过程都表现为形态表型。该协议描述了一种简单而强大的方法，用于分析完整的树突状乔木及其相关的棘。

Tags

CLARITY Neuronal Dendrites 3D Imaging Neuronal Connectivity Spatial Organization Electrophoresis Refractive Index Matching Microscopy

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