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Profilering van H3K4me3 Modificatie in Planten met behulp van Splitsing onder Doelen en Tagmentatie
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JoVE 신문 생물학
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
DOI:

09:48 min

April 22, 2022

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Chapters

  • 00:06Introduction
  • 01:51Nuclei Isolation
  • 04:22Antibody Incubation
  • 06:15Transposase Incubation
  • 07:24Tagmentation
  • 07:52DNA Extraction and NGS Library Construction
  • 08:19Representative Results
  • 09:25Conclusion

Summary

자동 번역

Splitsing onder doelen en tagmentatie (CUT&Tag) is een efficiënte chromatine epigenomische profileringsstrategie. Dit protocol presenteert een verfijnde CUT&Tag strategie voor de profilering van histonmodificaties in planten.

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