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Chapters
- 00:00Introduction
- 01:05hCF Dissociation
- 03:26CM Dissociation
- 05:59Collagen Preparation
- 06:59Hydrogel Mixture and Cell Preparation
- 07:41Device Insertion
- 09:32Results: Cardiac Tissue Formation
- 10:13Conclusion
The goal of this protocol is to explain and demonstrate the development of a three-dimensional (3D) microfluidic model of highly aligned human cardiac tissue, composed of stem cell-derived cardiomyocytes co-cultured with cardiac fibroblasts (CFs) within a biomimetic, collagen-based hydrogel, for applications in cardiac tissue engineering, drug screening, and disease modeling.
