/

/

人类MK从人类主要来源的免疫细胞分拣或血细胞遗传基因 的体外 分化

JoVE 신문
생물학
Author Produced

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Please note that all translations are automatically generated. Click here for the English version.

人类MK从人类主要来源的免疫细胞分拣或血细胞遗传基因的体外分化

DOI:

10.3791/62569-v

•

14:30 min

•

August 07, 2021

•

Andrea Acebes-Huerta, Patricia Martínez-Botía, Cristina Martín Martín, Ángel Bernardo³, Adrián R. Rodríguez, María Carmen Vicente-Ayuso, Celina Benavente Cuesta, Laura Gutiérrez⁶

¹Platelet Research Lab,Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ²Flow Cytometry and Cell Sorting Platform (ISPA), ³Clinical Diagnosis Laboratory – Dept. of Hematology,Hospital Universitario Central de Asturias (HUCA), ⁴Department of Hematology,Hospital Universitario Severo Ochoa (HUSO), ⁵Department of Hematology,Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), ⁶Dept. of Medicine,University of Oviedo

Chapters

00:00Introduction
01:17Background
02:12Methods: Immunophenotyping
03:50Results
06:58Relevance
07:42Methods: Cell Sorting
12:27Results and Post-Sort Analysis
12:53Limitations and Perspective

Summary

자동 번역

在这里，我们提出了一个免疫嗜血策略，用于巨型胡萝卜细胞分化的特征，并展示该策略如何允许使用荧光激活细胞分拣器在不同阶段对巨型胡萝卜细胞进行排序。该方法可以应用于人体原生组织，也适用于体外培养产生的巨型核细胞。

Tags

Immunophenotyping Cell Sorting Human Megakaryocytes Hematopoietic Progenitors Flow Cytometry Megakaryopoiesis Polyploidy Surface Markers Multi-omics Studies Platelet Production

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

人类胚胎干细胞：启动文化冷冻细胞

主电穿孔的小鼠骨髓造血细胞培养制备

从外植体生长的首要人权支气管上皮细胞

高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞

表型分析和分离小鼠造血干细胞和传承的承诺祖

天真成人干细胞的分离从初级人类成纤维细胞培养

从小鼠和人类结肠组织初级肌成纤维细胞的分离

从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

体外胰腺器官从分散的小鼠胚胎祖细胞

从人类肌肉提比利斯前部高级隔间收集骨骼肌肉活检，用于机械评估

Read Article