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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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JoVE 신문 유전학
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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14:56 min

May 06, 2022

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Chapters

  • 00:03Introduction
  • 00:43Day 1: Bisulfite Treatment
  • 01:21Day 2: Amplification – Methylation Assay, Manual Protocol
  • 02:52Day 3: Hybridization – Methylation Assay, Manual Protocol
  • 06:39Day 4: Staining – Methylation Assay, Manual Protocol
  • 10:48Representative Results
  • 14:34Conclusion

Summary

자동 번역

The present study describes the workflow to manage DNA methylation data obtained by microarray technologies. The protocol demonstrates steps from sample preparation to data analysis. All procedures are described in detail, and the video shows the significant steps.

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