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1Department of Internal Medicine, Ribeirão Preto Medical School,University of São Paulo, 2Centre for Computational Biology,University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine,The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport,University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School,University of Sao Paulo, 7Faculty of Pharmaceutical Sciences,University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School,University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto,University of São Paulo
Chapters
- 00:03Introduction
- 00:43Day 1: Bisulfite Treatment
- 01:21Day 2: Amplification – Methylation Assay, Manual Protocol
- 02:52Day 3: Hybridization – Methylation Assay, Manual Protocol
- 06:39Day 4: Staining – Methylation Assay, Manual Protocol
- 10:48Representative Results
- 14:34Conclusion
Bu çalışma, mikroarray teknolojileri tarafından elde edilen DNA metilasyon verilerini yönetmek için iş akışını açıklamaktadır. Protokol, numune hazırlamadan veri analizine kadar olan adımları gösterir. Tüm prosedürler ayrıntılı olarak açıklanmıştır ve video önemli adımları göstermektedir.
