JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 00:42Virus Acquisition, Collection of Viruliferous Insects, and Reagent Preparation
- 01:24Dissection of Adults and Excision of Guts
- 02:38Dissection of Nymphs and Excision of Guts
- 03:47Labeling Protocols for SRBSDV Virions and an Insect Protein
- 05:33Results: Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
- 06:32Conclusion
Dit protocol voor immunofluorescente etikettering van zowel plantaardige viruseiwitten als vectorinsecteneiwitten in weggesneden insectendarmen kan worden gebruikt om interacties tussen virus- en vectorinsecten, insecteneiwitfuncties en moleculaire mechanismen die ten grondslag liggen aan virusoverdracht te bestuderen.
