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An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth
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An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth

An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth

DOI:
10.3791/62617-v

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10:38 min

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September 22, 2021

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, , , , , , ,
1Department of Biochemistry and Molecular Biology,Drexel University College of Medicine, 2Department of Pharmacology and Physiology,Drexel University College of Medicine, 3Department of Radiation Oncology,Thomas Jefferson University, 4Sidney Kimmel Cancer Center,Thomas Jefferson University

Chapters

  • 00:04Introduction
  • 00:54Intracranial Tumor Cell Injection
  • 03:44Brain Slice Generation
  • 06:04IVIS Imaging of Slices
  • 07:40Results: Ex vivo Breast Cancer Brain Metastatic Tumor Growth
  • 10:08Conclusion

Summary

자동 번역

자동 번역

We introduce a protocol for measuring real-time drug and radiation response of breast cancer brain metastatic cells in an organotypic brain slice model. The methods provide a quantitative assay to investigate the therapeutic effects of various treatments on brain metastases from breast cancer in an ex vivo manner within the brain microenvironment interface.

Tags

Brain MetastasisEx Vivo Brain Slice ModelBreast CancerDrug Efficacy TestingTumor GrowthBrain-tumor-host InteractionsBrain Tumor Microenvironment

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