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使用膜片钳技术研究线粒体的产热能力
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The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria
DOI:

11:05 min

May 03, 2021

Chapters

  • 00:04Introduction
  • 01:06Mitochondrial Isolation from the Mouse Heart
  • 03:01Preparation of Mitoplasts with a French Press for Mechanical Rupture of the OMM
  • 04:07Electrophysiological Recordings of H+ Leak Through UCP1 and AAC
  • 08:20Results: The Analysis of FA-Dependent Proton Current via UCP1 in Brown Fat and via AAC in Heart Mitoplast
  • 10:05Conclusion

Summary

자동 번역

该方法文章详细介绍了使用膜片钳技术测量穿过线粒体内膜的H + 泄漏的主要步骤,这是一种研究线粒体产热能力的新方法。

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