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1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology,Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education,Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research,Yikon Genomics Co. Ltd.
Chapters
- 00:04Introduction
- 01:24Preparation and Denudation of Granulosa Cells, Oocyte Evaluation and Embryo Washing
- 03:15Culture Medium Collection Options 1 and 2, Sample Collection
- 05:12Culture Medium Lysis
- 06:22Pre‐Library Preparation
- 07:24Library Preparation
- 08:14Library Purification and Quantification, Sequencing and Data Analysis
- 09:43Results: Embryo Screening and Selection Using NICS from a Patient
- 11:08Conclusion
El presente estudio reporta un protocolo para el tamizaje cromosómico de embriones humanos que utiliza medio de cultivo gastado, lo que evita la biopsia embrionaria y permite la identificación de ploidía cromosómica mediante NGS. El presente artículo presenta el procedimiento detallado, que incluye la preparación del medio de cultivo, la amplificación del genoma completo (WGA), la preparación de la biblioteca de secuenciación de nueva generación (NGS) y el análisis de datos.
