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1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology,Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education,Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research,Yikon Genomics Co. Ltd.
Chapters
- 00:04Introduction
- 01:24Preparation and Denudation of Granulosa Cells, Oocyte Evaluation and Embryo Washing
- 03:15Culture Medium Collection Options 1 and 2, Sample Collection
- 05:12Culture Medium Lysis
- 06:22Pre‐Library Preparation
- 07:24Library Preparation
- 08:14Library Purification and Quantification, Sequencing and Data Analysis
- 09:43Results: Embryo Screening and Selection Using NICS from a Patient
- 11:08Conclusion
La présente étude fait état d’un protocole de criblage chromosomique d’embryons humains qui utilise un milieu de culture épuisé, ce qui évite la biopsie embryonnaire et permet l’identification de la ploïdie chromosomique à l’aide du NGS. Le présent article présente la procédure détaillée, y compris la préparation du milieu de culture, l’amplification du génome entier (WGA), la préparation de la bibliothèque de séquençage de nouvelle génération (NGS) et l’analyse des données.
