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一种人外周血淋巴细胞γ-H2AX病灶测定的自动显微镜评分方法
JoVE 신문
암 연구학
Author Produced
This content is Free Access.
JoVE 신문 암 연구학
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
DOI:

08:23 min

December 25, 2021

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Chapters

  • 00:00Introduction
  • 00:44Preparation of Samples
  • 01:26Sample Irradiations
  • 01:35Slide Preparation
  • 02:17Fixation and Immunofluorescence γ-H2AX Staining
  • 03:44Automated Metafer Scanning and Scoring
  • 07:29Results
  • 08:03Conclusion

Summary

자동 번역

该协议介绍了外周血淋巴细胞上γ-H2AX病灶测定的载玻片制备和自动评分。为了说明测定的方法和灵敏度,在 体外照射分离的淋巴细胞。这种自动化的DNA DSB检测方法对于快速和高通量的生物剂量测定应用非常有用。

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