AAV Deployment of Enhancer-Based Expression Constructs <em>In Vivo</em> in Mouse Brain

00:04Introduction
00:39Enhancer Candidate Sequence Cloning into the AAV Vector Plasmid
03:09Intracranially Inject rAAV-Packaged Plasmid into Neonatal Mice
05:32Image and Analyze Brain Tissue Sections for Enhancer Activity
08:10Results: AAV Deployment of Enhancer-Based Expression Constructs in Mouse Brain
09:21Conclusion

Summary

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This protocol describes a novel rAAV-based transient enhancer-reporter assay. This assay can be used to induce enhancer-driven expression in vivo in the mouse brain.

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

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