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无需梯度测定器或分馏系统的多聚体分析

JoVE 신문
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JoVE 신문 생화학

Polysome Profiling without Gradient Makers or Fractionation Systems

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无需梯度测定器或分馏系统的多聚体分析

DOI:

10.3791/62680-v

•

05:56 min

•

June 01, 2021

•

Mack Sobhany, Robin E. Stanley

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Chapters

00:04Introduction
00:39Preparation of 7 to 47% Sucrose Gradients
02:14Preparation of Yeast Cell Extracts
03:21Centrifugation of Gradients
03:49Fraction and Data Collection
04:36Results: Polysome Profiling Without Gradient Makers or Fractionation Systems
05:19Conclusion

Summary

자동 번역

该协议描述了如何在不使用自动梯度制作器或梯度分级分离系统的情况下生成多聚体谱。

Tags

Polysome Profiling Gradient-free Sucrose Gradient Ribosome Activity Yeast Cell Lysis RNA Extraction RNA Quantification

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