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Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
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JoVE 신문 신경과학
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
DOI:

08:31 min

July 29, 2021

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Chapters

  • 00:00Introduction
  • 01:13Microscope Setup, Loading and Imaging ND6 in Neuronal Cultures
  • 04:17Quantification of Membrane Trafficking by a Change in ND6 Fluorescence
  • 06:44Results: Membrane Lipid Turnover with the pH‐Sensitive Fluorescent Lipid Analog ND6
  • 08:00Conclusion

Summary

자동 번역

This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.

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