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Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
JoVE 신문
생물학
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JoVE 신문 생물학
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
DOI:

07:29 min

August 13, 2021

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Chapters

  • 00:04Introduction
  • 00:52Preparation of Zebrafish
  • 01:51Fluorinated Ethylene Propylene (FEP) Tubes Preparation
  • 02:542% Agarose Dish and Embedding Media Preparation
  • 03:29Sample Mounting
  • 05:03Sample Positioning
  • 05:40Image Acquisition and Processing
  • 06:13Results: Imaging of the Developing Heart
  • 06:54Conclusion

Summary

자동 번역

We describe optimized tools to study the zebrafish heart in vivo with light sheet fluorescence microscopy. Specifically, we suggest bright cardiac transgenic lines and present new gentle embedding and immobilization techniques that avoid developmental and heart defects. A possible data acquisition and analysis pipeline adapted to cardiac imaging is also provided.

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