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活植物细胞中的Förster共振能量转移测量
JoVE 신문
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JoVE 신문 생화학
Förster Resonance Energy Transfer Measurements in Living Plant Cells
DOI:

06:53 min

June 28, 2021

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Chapters

  • 00:04Introduction
  • 00:25Laser Adjustment
  • 02:03Adjustment of Photomultipliers
  • 02:57Frster Resonance Energy Transfer (FRET) Image Acquisition
  • 04:09Determination of Crosstalk Corrections, Calibration of the Measurements, and Data Evaluation
  • 05:13Results: FRET Measurements in Living Plant Cells
  • 06:22Conclusion

Summary

자동 번역

提供了一个方案,用于设置标准的共聚焦激光扫描显微镜,用于 体内 Förster共振能量转移测量,然后进行数据评估。

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