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1Department of Biological Sciences,Ohio University, 2Molecular and Cellular Biology Program,Ohio University, 3Neuroscience Program,Ohio University, 4Edison Biotechnology Institute,Ohio University, 5Translational Biomedical Sciences Program,Ohio University, 6Honors Tutorial College,Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine,Ohio University
Chapters
- 00:05Introduction
- 01:05Tumor Cell Culture and Xenograft Tumor Development
- 02:41HMWFD and NHF-ATP Solution Preparation
- 03:21ATP Internalization in Tumor Xenograft
- 03:53Cryo-embedding
- 04:45Tissue Slide Fixation
- 05:25Fluorescence Microscopy and Image Acquisition
- 07:12Results: Assessment of Nonhydrolyzable Fluorescent ATP Internalization
- 07:44Conclusion
Desenvolvemos um método reprodutível para visualizar a internalização do triphosfato fluorescente nãohydrolyzável (ATP), um substituto da ATP, com alta resolução celular. Validamos nosso método usando linhas celulares tumorais in vitro e in vivo e camundongos imunodeficentes xenoenxerados com tecido tumoral humano.
