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Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
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생물학
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Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

DOI:
10.3791/62790-v

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08:17 min

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August 16, 2021

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, , ,
1NEST Laboratory,Scuola Normale Superiore, 2Center for Nanotechnology Innovation@NEST CNI@NEST

Chapters

  • 00:04Introduction
  • 00:58Sample Preparation
  • 02:35Data Acquisition
  • 03:52iMSD Calculation
  • 05:54Results: Structural and Dynamic Properties of Lysosomes and Macropinosomes
  • 07:45Conclusion

Summary

자동 번역

자동 번역

Imaging-derived mean square displacement (iMSD) analysis is applied to macropinosomes to highlight their intrinsic time-evolving nature in terms of structural and dynamic properties. Macropinosomes are then compared to insulin secretory granules (ISGs) as a reference for subcellular structures with time-invariant average structural/dynamic properties.

Tags

Keywords: Trafficking Subcellular NanostructuresSpatiotemporal Fluctuation SpectroscopyImaging The Right Mean Square DisplacementSubcellular Nano-structuresCell LabelingTime-lapsed Series AcquisitionFluorescence ImagingEGFPMacropinesomes

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