Journal
/
생물학
/
从全血和巴菲皮脂中分离出人类嗜中性粒细胞
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Isolation of Human Neutrophils from Whole Blood and Buffy Coats
Please note that all translations are automatically generated. Click here for the English version.

从全血和巴菲皮脂中分离出人类嗜中性粒细胞

DOI:
10.3791/62837-v

•

08:12 min

•

September 17, 2021

•

, , ,
1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

Chapters

  • 00:04Introduction
  • 00:45Gradient Layering
  • 02:40Erythrocyte Sedimentation
  • 03:45Residual RBC Lysis
  • 05:06Quality Determination of Isolated Neutrophil
  • 05:44Results: Neutrophil Yield and Quality Estimation
  • 07:34Conclusion

Summary

자동 번역

자동 번역

该协议详细介绍了从全血，黄褐色涂层或白细胞分离膜中性粒细胞的方法，从而实现良好的产量，高纯度和最小的细胞活化。我们利用梯度纯化，红细胞（RBC）沉降和红细胞裂解来获得高质量/纯度的中性粒细胞制剂。

Tags

NeutrophilsDensity GradientSedimentationLysisBuffy CoatPBMCErythrocyteCell BiologyCell IsolationCell Preparation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/62837?article_type=v

NK细胞与干细胞技术的RosetteSep套件的人体血液的富集
check_url/kr/62837?article_type=v

从新生小鼠的肺血管内皮细胞的分离和培养
check_url/kr/62837?article_type=v

成人从人体皮肤的上皮干细胞的分离和培养
check_url/kr/62837?article_type=v

天真成人干细胞的分离从初级人类成纤维细胞培养
check_url/kr/62837?article_type=v

从人类Lipoaspirates脂肪来源干细胞的手动隔离
check_url/kr/62837?article_type=v

从小鼠和人类结肠组织初级肌成纤维细胞的分离
check_url/kr/62837?article_type=v

从人骨骼肌隔离血管源性多能前体
check_url/kr/62837?article_type=v

小非编码RNA从人血清中分离
check_url/kr/62837?article_type=v

从小鼠和人类食管隔离肌纤维母细胞
check_url/kr/62837?article_type=v

人外周血代文化血液的产物内皮细胞

Read Article