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Journal / Bioengineering / 使用无细胞自动诱导工作流程在24小时内从细胞到无细胞蛋白质合成…
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JoVE Journal Bioengineering
使用无细胞自动诱导工作流程在24小时内从细胞到无细胞蛋白质合成
 
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使用无细胞自动诱导工作流程在24小时内从细胞到无细胞蛋白质合成

Article DOI: 10.3791/62866-v • 06:08 min • July 22nd, 2021
July 22nd, 2021 •
1,2, 1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, California Polytechnic State University San Luis Obispo, 2Center for Application in Biotechnology, California Polytechnic State University San Luis Obispo

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这项工作描述了从 大肠杆菌（E. coli） 制备细胞提取物，然后在24小时内进行无细胞蛋白质合成（CFPS）反应。无细胞自诱导（CFAI）方案的解释详细介绍了为减少研究人员的监督和增加获得的细胞提取物数量而进行的改进。

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生物工程， 第173期，
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