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使用短读和长读测序技术生成尿细菌完整基因组的杂交从头基因组组装

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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使用短读和长读测序技术生成尿细菌完整基因组的杂交从头基因组组装

DOI:

10.3791/62872-v

•

12:08 min

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August 20, 2021

•

Belle M. Sharon, Neha V. Hulyalkar, Vivian H. Nguyen, Philippe E. Zimmern, Kelli L. Palmer, Nicole J. De Nisco

¹Department of Biological Sciences,University of Texas at Dallas, ²Department of Urology,University of Texas Southwestern Medical Center

Chapters

00:04Introduction
01:00dsDNA Pool Cleanup and Concentration
03:25Sequence the Genome Library
05:14Long (MinION) Reads
06:55Generating Hybrid Genome Assembly
08:34Assessing Assembly Quality
09:34Genome Annotation
10:07Results: Analysis of Extracted DNA and Complete Genome Sequencing of Urinary Bacteria
11:37Conclusion

Summary

자동 번역

该协议详细介绍了尿细菌培养，测序和从头杂交基因组组装的综合方法。它为生成完整的圆形基因组序列提供了一种可重复的程序，可用于研究有助于尿定植、发病机制和抗菌素耐药性传播的染色体和染色体外遗传元件。

Tags

Keywords: Hybrid Genome Assembly Urinary Bacteria Short-read Sequencing Long-read Sequencing Complete Genomes Genome Architecture Mobile Genetic Elements Culturable Bacteria Genome Content Adapter Ligation Flow Cell Priming DNA Library Preparation Sequencing Run

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