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分析测定切除实体瘤匀浆物的线粒体功能
JoVE 신문
암 연구학
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JoVE 신문 암 연구학
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
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分析测定切除实体瘤匀浆物的线粒体功能

DOI:
10.3791/62875-v

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11:32 min

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August 06, 2021

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, , ,
1Integrated Physiology and Molecular Medicine Laboratory,Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism,Pennington Biomedical Research Center, 3Department of Nutrition,Case Western Reserve University, 4Department of Clinical Nutrition,University of Texas Southwestern Medical Center

Chapters

  • 00:04Introduction
  • 00:54Tumor Homogenate Preparation
  • 04:27Substrate, Uncoupler, Inhibitor Titration Protocol (SUIT)
  • 09:00Results: Mitochondrial Function of Excised Solid Tumors Homogenates
  • 10:52Conclusion

Summary

자동 번역

자동 번역

我们开发了一种实用的方案和分析方法来评估新鲜肿瘤匀浆中的线粒体氧化磷酸化和电子转移能力。该协议可以很容易地用于调查有助于癌症发生，进展和治疗反应的各种线粒体功能。

Tags

Mitochondrial FunctionTumor HomogenatesMitochondrial RespirationBiopsy PreservationQuantitative AssessmentClinical Translational OncologyDiagnostic InterpretationCancer Progression

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