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Immunocoloration flottante libre du cerveau des souris
JoVE 신문
신경과학
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JoVE 신문 신경과학
Free-floating Immunostaining of Mouse Brains
DOI:

07:58 min

October 07, 2021

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Chapters

  • 00:04Introduction
  • 00:50Perfusion
  • 02:57Cryosectioning
  • 04:06Free-Floating Wisteria Florbunda Agglutinin Staining and Anti- Arginine Vasopressin Immunostaining
  • 05:13Mounting
  • 06:00Imaging
  • 06:42Results: Double Immunofluorescent Staining of Free-Floating Mouse Brain Sections
  • 07:21Conclusion

Summary

자동 번역

Ce protocole décrit une approche efficace et reproductible pour les études histologiques du cerveau de souris, y compris la perfusion, la section du cerveau, l’immunocoloration flottante, le montage tissulaire et l’imagerie.

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