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1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
Chapters
- 00:04Introduction
- 00:38SPECT Camera Preparation and Calibration
- 01:47[125I]CLINDE Radiotracer Synthesis
- 04:27SPECT Acquisition
- 05:15Scan Reconstruction
- 05:57Rat Brain Extraction and Cell Isolation
- 09:46Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
- 11:27Conclusion
Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) est un outil puissant pour étudier le rôle de la protéine translocatrice 18 kDa ou de l’expression du récepteur 5HT2A de la sérotonine dans la maladie d’Alzheimer à l’échelle cellulaire. Ce protocole décrit l’application ex vivo de FACS-RTT dans le modèle de rat TgF344-AD.
