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Chapters
- 00:04Introduction
- 01:00Polyplexes Formation, Lyophilization, and Resuspension
- 02:44In Vitro Characterization
- 04:34In Vitro Functionality Tests: Capacity to Activate Model Immune Cells by Using Ovalbumin as Antigenic Model mRNA
- 06:24Results: Nanoparticle Synthesis and Physicochemical Characterization, mRNA Encapsulation Efficiency, and In Vitro Results
- 07:45Conclusion
Here, a simple protocol is presented for producing mRNA nanoparticles based on poly(beta aminoester) polymers, easy to be tailored by changing the encapsulated mRNA. The workflow for synthesizing the polymers, the nanoparticles, and their in vitro essential characterization are also described. A proof-of-concept regarding immunization is also added.
