Journal
/
생물학
/
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

DOI:
10.3791/62893-v

•

07:28 min

•

October 04, 2021

•

, , ,
1Department of Biodiversity and Molecular Ecology, Research and Innovation Centre,Fondazione Edmund Mach

Chapters

  • 00:04Introduction
  • 00:46Reagents and Media Preparation
  • 01:59Aspirator Setup
  • 02:37High-Throughput Liquid Surface Sterilization of Seeds
  • 04:56Plating and Scoring of Arabidopsis on Half Strength Murashige and Skoog Plates
  • 05:32Results: Germination Analysis of Arabidopsis Seeds After Surface Sterilization with the New Method
  • 06:57Conclusion

Summary

자동 번역

자동 번역

A high-throughput protocol for the surface sterilization of Arabidopsisthaliana (Arabidopsis) seeds is provided, optimizing the liquid handling steps with a simple suction device constructed with a vacuum pump. Hundreds of seed samples can be surface-sterilized in one day.

Tags

Keywords: ArabidopsisSeed Surface SterilizationHigh-throughputRobustTime-flexibleFunctional GenomicsModel Plant SpeciesEthanolBleachMurashige And Skoog MediumAgarKanamycinAspiratorVacuum PumpDecantation BottlePolyethylene TubeAquarium Valve

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/62893?article_type=v

A high-throughput method to globally study the organelle morphology in S. cerevisiae
check_url/kr/62893?article_type=v

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
check_url/kr/62893?article_type=v

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
check_url/kr/62893?article_type=v

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
check_url/kr/62893?article_type=v

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
check_url/kr/62893?article_type=v

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
check_url/kr/62893?article_type=v

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
check_url/kr/62893?article_type=v

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
check_url/kr/62893?article_type=v

Recovery of Adult Zebrafish Hearts for High-throughput Applications
check_url/kr/62893?article_type=v

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Read Article