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Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro
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Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

DOI:
10.3791/62913-v

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08:05 min

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October 13, 2021

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, , ,
1Department of Anesthesiology,Vanderbilt University Medical Center, 2Anesthesiology,TVHS VA Medical Center, 3Department of Pharmacology,Vanderbilt University

Chapters

  • 00:04Introduction
  • 00:50Experimental Preparation/Plating
  • 02:58Hypoxia/Reoxygenation to Simulate Ischemia/Reperfusion Injury In Vitro
  • 04:28Endpoint Assessment
  • 05:17Results: Effect of Three Types of Culture Inserts and Different Concentrations of Co-Cultured Endothelial Cells on Assessment of the Release of Lactate Dehydrogenase (LDH)
  • 07:36Conclusion

Summary

자동 번역

자동 번역

Spatial distance is a key parameter in assessing hypoxia/reoxygenation injury in a co-culture model of separate endothelial and cardiomyocyte cell layers, suggesting, for the first time, that optimizing the co-culture spatial environment is necessary to provide a favorable in vitro model for testing the role of endothelial cells in cardiomyocyte protection.

Tags

Cell Co-cultureCardiac Ischemia/reperfusionIn Vitro ModelCardiomyocyteEndothelial CellSpatial EnvironmentCell-cell InteractionsHypoxia Reperfusion InjuryCell CultureCell Seeding DensityExtracellular Matrix

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