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Journal / Neuroscience / 使用遗传编码的Ca2 + 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca2 +…
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JoVE Journal Neuroscience
使用遗传编码的Ca2 + 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca2 + 摄取进行成像
 
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使用遗传编码的Ca2 + 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca2 + 摄取进行成像

Article DOI: 10.3791/62917-v • 07:46 min • January 22nd, 2022
January 22nd, 2022 •
1, 1,2, 2, 1,2
1Dalton Cardiovascular Research Center, University of Missouri-Columbia, 2Department of Biomedical, Biological and Chemical Engineering, University of Missouri-Columbia

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该proctocol旨在为星形胶质细胞和神经元 中的体外 和 体内 线粒体Ca2 + 成像提供一种方法。

Tags

神经科学，第179期，星形胶质细胞，神经元，线粒体，GECI，GCaMP5G / 6s，线粒体靶向序列，gfaABC1D启动子，CaMKII启动子， 体内 2光子成像
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