Journal
/
/
Limpieza selectiva de nematodos silvestres de Caenorhabditis para enriquecer las bacterias del microbioma intestinal
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
DOI:

09:47 min

August 13, 2021

, , ,

Chapters

  • 00:04Introduction
  • 00:34Inducing Dauer Formation for Wild Nematodes on Nematode Growth Medium (NGM) Plates
  • 01:16Washing and Disinfecting the Nematodes
  • 03:12Removing the Antibiotic and SDS Solution
  • 04:08Propagate a Clean Nematode Strain
  • 05:27Intestinal Dissection and PCR Identification of Microbial Species
  • 08:17Results: The Host-microbe Interactions in the Context of the Intestines of Caenorhabditis tropicalis Strain JU1848
  • 09:16Conclusion

Summary

자동 번역

Los nematodos de Caenorhabditis silvestre están asociados con muchos microbios, a menudo en la luz intestinal o infectando el intestino. Este protocolo detalla un método para enriquecer microbios inculturables que colonizan el intestino, aprovechando la resistencia de la cutícula dauer.

Related Videos

Read Article