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Live-imaging van mitochondriaal systeem in gekweekte astrocyten
JoVE 신문
신경과학
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JoVE 신문 신경과학
Live-imaging of Mitochondrial System in Cultured Astrocytes
DOI:

06:20 min

November 16, 2021

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Chapters

  • 00:04Introduction
  • 00:44Rat Hippocampal Astrocyte Primary Culture
  • 01:25Long-Term Monitoring of the Mitochondrial System
  • 02:13Analysis of Individual Mitochondrial Morphology and LV-G1-MitoTimer Ratio
  • 03:54Analysis of Mitochondrial Motility
  • 04:28Data Transformation, Normalization, and Statistical Analysis
  • 04:51Results: Analysis of the Effects of H2O2 on the Mitochondrial System of Astrocytes
  • 05:53Conclusion

Summary

자동 번역

Dit artikel beschrijft de methode voor mitochondriale time-lapse beeldvorming van astrocytenculturen uitgerust met MitoTimer biosensor en de resulterende multiparametrische analyse van mitochondriale dynamica, mobiliteit, morfologie, biogenese, redoxtoestand en omzet.

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