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1A.N. Belozersky Institute of Physico-Chemical Biology,Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine,Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Chapters
- 00:05Introduction
- 00:44Protocol
- 01:02Obtaining a Primary Culture of Human Skin Fibroblasts
- 02:14Cell Cultivation
- 04:45Transfection
- 06:46Preparing for Imaging
- 08:08Setting the Imaging Parameters
- 09:45Results
- 10:13Conclusion
Ce protocole est dédié à la visualisation des microtubules par transfection de la protéine EB3 afin d’étudier leurs propriétés dynamiques en culture cellulaire primaire. Le protocole a été mis en œuvre sur des fibroblastes cutanés primaires humains obtenus chez des patients atteints de la maladie de Huntington.
