Live-Cell Imaging van transcriptionele activiteit bij DNA Double-Strand Breaks

Article DOI: 10.3791/62968-v • 09:07 min • September 20th, 2021

September 20th, 2021 •

Madalena R. de Almeida¹, Eduardo Gameiro¹, Sérgio F. de Almeida¹, Robert M. Martin¹

¹Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa

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Dit protocol presenteert een nieuw reporter-gensysteem en de experimentele opstelling om transcriptie te detecteren bij DNA-dubbelstrengsbreuken met gevoeligheid van één molecuul.

Live-Cell Imaging van transcriptionele activiteit bij DNA Double-Strand Breaks

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