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两种剥离方法，用于分离小鼠视网膜中的光感受器细胞区室以进行蛋白质分析

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Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

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两种剥离方法，用于分离小鼠视网膜中的光感受器细胞区室以进行蛋白质分析

DOI:

10.3791/62977-v

•

11:08 min

•

December 07, 2021

•

Kasey Rose, Sowmya Lokappa, Jeannie Chen²

¹Zilkha Neurogenetic Institute, Keck School of Medicine,University of Southern California, ²Department of Cell & Neurobiology, Keck School of Medicine,University of Southern California

Chapters

00:04Introduction
00:52Live Cell Retinal Peeling Method
03:35Lyophilized Retina Peeling Method
07:42Results: Isolation of Subcellular Compartments of Murine Rod Photoreceptors for Protein Analysis
10:23Conclusion

Summary

자동 번역

该协议提出了两种技术来分离小鼠杆光感受器的亚细胞区室以进行蛋白质分析。第一种方法利用活视网膜和纤维素滤纸来分离杆外段，而第二种方法采用冻干视网膜和胶带剥离杆内段和外段层。

Tags

Photoreceptor Cell Compartments Mouse Retina Protein Analysis Peeling Techniques Rod Subcellular Compartments Rod Outer Segment (ROS)Ames HEPES Buffer Filter Paper C57 Black 6J Mouse Retinal Dissection Protein Analysis

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