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Une boîte à outils de transcription-traduction de streptomyces à haut rendement pour la biologie synthétique et les applications de produits naturels
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JoVE 신문 생체공학
A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
DOI:

07:59 min

September 10, 2021

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Chapters

  • 00:05Introduction
  • 00:58Preparation of Streptomyces venezuelae ATCC 10712 Cells
  • 03:56Cell Lysis by Sonication
  • 04:47Cell Extract Clarification and Run-off Reaction
  • 05:14Set-up a Standard Streptomyces venezuelae Transcription-Translation (TX-TL) Reaction
  • 06:21Results: Streptomyces Transcription Translation Toolkit with the pTU1-A-SP44-mScarlet-I Plasmid
  • 07:18Conclusion

Summary

자동 번역

Ce protocole détaille une méthode améliorée pour synthétiser des rendements élevés de protéines recombinantes à partir d’un système de transcription-traduction sans cellule (TX-TL) de Streptomyces venezuelae .

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