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Immunology and Infection

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JoVE Journal Immunology and Infection
Het genereren van transgenen en knock-outs in strongyloides soorten door micro-injectie
 
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Het genereren van transgenen en knock-outs in strongyloides soorten door micro-injectie

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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De functionele genomische toolkit voor de parasitaire nematoden Strongyloides stercoralis en Strongyloides ratti omvat transgenese, CRISPR/Cas9-gemedieerde mutagenese en RNAi. Dit protocol zal demonstreren hoe intragonadale micro-injectie kan worden gebruikt om transgenen en CRISPR-componenten in S. stercoralis en S. ratti te introduceren.

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Immunologie en infectie nummer 176
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