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ืืฆืืจืช ืืืืจืื ืืืื ืืกืื ืื ืืงืืืืืื ืืืื ืื ืกืืจืื ืืืืืืืืื ืขื ืืื ืืืงืจื-ืืื ืืืจืฆืื
JoVE ๋น๋์ค๋ฅผ ํ์ฉํ์๋ ค๋ฉด ๋์๊ด์ ํตํ ๊ธฐ๊ด ๊ตฌ๋
์ด ํ์ํฉ๋๋ค. ์ ์ฒด ๋น๋์ค๋ฅผ ๋ณด์๋ ค๋ฉด ๋ก๊ทธ์ธํ๊ฑฐ๋ ๋ฌด๋ฃ ํธ๋ผ์ด์ผ์ ์์ํ์ธ์.
Chapters
- 00:05Introduction
- 00:37Preparation of Microinjection Slides
- 01:19Collection of Young Adult Strongyloides for Microinjection
- 03:19Preparing the Microscope and Breaking the Needle
- 05:27Microinjecting Strongyloides
- 07:22Collecting and Screening F1 Larvae to Recover Transgenics/Knockouts
- 08:31Results: Generating Transgenics and Knockouts in Strongyloides Species Using Intragonadal Microinjection
- 09:04Conclusion
ืขืจืืช ืืืืื ืืื ืืืืช ืืคืื ืงืฆืืื ืืืช ืขืืืจ ืื ืืืืืืช ืืืคืืืืืช ืกืืจืื ืืืืืืืืื ืกืืจืงืืจืืืก ืืกืืจืืื ืืืืืืื ืจืืื ืืืืืช ืืจื ืกืื ืื, ืืืืื ืื ืืชืืืื CRISPR/Cas9 ื-RNAi. ืคืจืืืืงืื ืื ืืืืื ืืืฆื ืืืฉืชืืฉ ืืืืงืจื-ืืื ืื'ืงืฆืื ืชืื-ืืื ืืืืช ืืื ืืืื ืืก ืืจื ืกื'ื ืื ืืจืืืื ืงืจืืกืคืจ ืืชืื S. stercoralis ื - S. ratti.
