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从小鼠和人类牙齿中快速分离单个细胞

JoVE 신문
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JoVE 신문 발생학

Rapid Isolation of Single Cells from Mouse and Human Teeth

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从小鼠和人类牙齿中快速分离单个细胞

DOI:

10.3791/63043-v

•

06:09 min

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October 28, 2021

•

Jan Krivanek, Josef Lavicky, Thibault Bouderlique, Igor Adameyko³

¹Department of Histology and Embryology, Faculty of Medicine,Masaryk University, ²Department of Molecular Neuroimmunology, Centre for Brain Research,Medical University of Vienna, ³Department of Physiology and Pharmacology,Karolinska Institute

Chapters

00:05Introduction
01:09Tissue Dissection
02:58Single-Cell Suspension Preparation
04:42Results: Quantification of the Immune Cells
05:24Conclusion

Summary

자동 번역

目前的方案提出了一种快速、高效和温和的方法，用于从持续生长的小鼠门牙、小鼠臼齿和人类牙齿中分离出适合单细胞RNA-seq分析的单细胞。

Tags

Single-cell Isolation Mouse Teeth Human Teeth Cell Suspension Dental Pulp Mandible Euthanasia HBSS Tweezers Scalpel Cell Quality

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