JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Pathology,University of Michigan, Ann Arbor, 2Department of Internal Medicine,University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology,University of Michigan, Ann Arbor, 4Rogel Cancer Center,University of Michigan, Ann Arbor, 5Institute of Gerontology,University of Michigan, Ann Arbor
Chapters
- 00:05Introduction
- 01:08Hydration of the Sensor Cartridge
- 02:03Preparation of Cell-Adhesive-Coated Microplates
- 02:52Seeding Cells in Cell-Adhesive-Coated Microplates
- 04:10Glycolysis Stress Test Using the Extracellular Flux Analyzer
- 07:53Results: Assessment of the Glycolytic Function and Mitochondrial Respiration in Mouse HSPCs
- 09:36Conclusion
The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.
