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免疫肽组学：分离小鼠和人MHC I类和II类相关肽用于质谱分析
JoVE 신문
생화학
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JoVE 신문 생화학
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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免疫肽组学：分离小鼠和人MHC I类和II类相关肽用于质谱分析

DOI:
10.3791/63052-v

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09:32 min

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October 15, 2021

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, , , ,
1CHU Sainte-Justine Research Center, 2Department of Pathology and Cellular Biology, Faculty of Medicine,Université de Montréal

Chapters

  • 00:04Introduction
  • 00:41Coupling Antibodies to Sepharose CNBr-Activated Beads
  • 02:57Cell Lysis and Immunocapture with Antibody Coupled-Beads
  • 04:06Elution of MHC Peptides
  • 07:11Results: Analysis of the MHC-Binding Affinity
  • 08:56Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了一种从小鼠和人细胞系纯化MHC I类和II类肽复合物的方案，提供高质量的免疫肽组学数据。该协议侧重于使用市售抗体进行样品制备。

Tags

ImmunopeptidomicsMHC Class IMHC Class IIPeptide IsolationMass SpectrometryHumanMouseAutoimmune DiseasesCancerVirologySepharose BeadsAntibody CouplingBlockingWashingCell LysisPeptide Elution

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